Hotspot

ycf3-trnS-GGA

LSC · 47426-48368 · 943 bp

Back to Fallopia

Pi 0.0394

MarkerSeek score 77.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 71
pi: 0.0394
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCGCCTGAAGTTGTTGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	58.0	57.8	1077-1086 bp	1.0	85.6
ycf3-trnS-GGA_p2	TTTCGCCTGAAGTTGTTGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	58.0	57.8	1078-1087 bp	1.0	85.6
ycf3-trnS-GGA_p3	TTGGCCACAACTGAAAAGGT	TTCCAATGCTACGCCTTGAA	45.0	45.0	58.1	57.8	1040-1049 bp	1.0	85.2
ycf3-trnS-GGA_p4	TCGCCTGAAGTTGTTGGAAT	TTCCAATGCTACGCCTTGAA	45.0	45.0	57.7	57.8	1076-1085 bp	1.0	83.9
ycf3-trnS-GGA_p5	GGCCACAACTGAAAAGGTCT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	1038-1047 bp	1.0	83.8