Hotspot

trnT-GGU-psbD

LSC · 34220-35716 · 1497 bp

Back to Fallopia

Pi 0.0349

MarkerSeek score 74.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1497
variable_sites: 88
pi: 0.0349
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	ACTTTACCAAGGGCTATAGTCA	50.0	40.9	58.3	56.3	1486-1555 bp	1.0	56.4
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	AACTTTACCAAGGGCTATAGTCA	50.0	39.1	58.3	57.0	1487-1556 bp	1.0	54.1
trnT-GGU-psbD_p3	GCCATGGTAAGGCGTAAGTC	ACTTTACCAAGGGCTATAGTCA	55.0	40.9	58.7	56.3	1496-1565 bp	1.0	53.0
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	ACTTTACCAAGGGCTATAGTCAT	50.0	39.1	58.3	56.8	1486-1555 bp	1.0	52.5
trnT-GGU-psbD_p5	AGAGTAACGCCATGGTAAGG	ACTTTACCAAGGGCTATAGTCA	50.0	40.9	57.0	56.3	1504-1573 bp	1.0	50.8