Hotspot

trnE-UUC-trnT-GGU

LSC · 33234-34147 · 914 bp

Back to Fallopia

Pi 0.0442
MarkerSeek score 79.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
914
variable_sites
76
pi
0.0442
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTTGTGAGAGCGACACGAA GAACCGATGACTTACGCCTT 45.0 50.0 58.1 58.0 1437-1465 bp 1.0 88.9
trnE-UUC-trnT-GGU_p2 TTTTTGTGAGAGCGACACGA GAACCGATGACTTACGCCTT 45.0 50.0 58.1 58.0 1438-1466 bp 1.0 88.9
trnE-UUC-trnT-GGU_p3 TTGTGAGAGCGACACGAATT GAACCGATGACTTACGCCTT 45.0 50.0 57.9 58.0 1435-1463 bp 1.0 88.3
trnE-UUC-trnT-GGU_p4 TGGATTTGAACCAGCGTAGG GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1176-1195 bp 1.0 87.4
trnE-UUC-trnT-GGU_p5 ATTTGAACCAGCGTAGGCAT GAACCGATGACTTACGCCTT 45.0 50.0 57.9 58.0 1173-1192 bp 1.0 87.3