Hotspot

rpoB-trnC-GCA

LSC · 27994-29354 · 1361 bp

Back to Fallopia

Pi 0.0387

MarkerSeek score 74.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1361
variable_sites: 93
pi: 0.0387
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCCCAGGTATTGTCGACATT	ACTCGGGGTTACTGAACAGA	50.0	50.0	58.0	58.0	1828-1906 bp	1.0	89.1
rpoB-trnC-GCA_p2	CCAAATTAACCCCGCGGATA	ACTCGGGGTTACTGAACAGA	50.0	50.0	58.0	58.0	2049-2127 bp	1.0	88.7
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	ACTCGGGGTTACTGAACAGA	50.0	50.0	58.0	58.0	2297-2375 bp	1.0	88.3
rpoB-trnC-GCA_p4	CCAAATTAACCCCGCGGATA	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	1710-1789 bp	0.75	77.6
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	CGAATCAAGCGCGTATCAAC	50.0	50.0	58.0	58.0	1958-2037 bp	0.75	77.3