Hotspot

ycf1

SSC · 127869-133544 · 5676 bp

Pi 0.00422

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 86
pi: 0.00422
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCCCACGGTTGAATCTTAT	GCCGATATCGATACCGTCAG	50.0	55.0	57.9	57.8	264 bp	1.0	80.6
ycf1_p2	TTTGATTGGTATGCCCCACG	GCCGATATCGATACCGTCAG	50.0	55.0	58.5	57.8	276 bp	1.0	76.7
ycf1_p3	GCCCCACGGTTGAATCTTAT	CCGATATCGATACCGTCAGG	50.0	55.0	57.9	57.2	263 bp	1.0	75.4
ycf1_p4	GTATGCCCCACGGTTGAATC	GCCGATATCGATACCGTCAG	55.0	55.0	59.0	57.8	268 bp	1.0	73.2
ycf1_p5	TCGGAAATTCTGGGAAGAACC	GCCGATATCGATACCGTCAG	47.6	55.0	57.9	57.8	229 bp	1.0	72.8