Hotspot

rpoC1

LSC · 21347-24189 · 2843 bp

Back to Fagus

Pi 0.002
MarkerSeek score 52.80
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2843
variable_sites
25
pi
0.002
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 CCCTAATTCACATCGGTGTCA ACGATCTTTGGCTTTGGAACT 47.6 42.9 57.7 58.1 2993-2999 bp 0.909091 57.9
rpoC1_p2 ACCCTAATTCACATCGGTGTC ACGATCTTTGGCTTTGGAACT 47.6 42.9 57.7 58.1 2994-3000 bp 0.909091 57.9
rpoC1_p3 ACCCTAATTCACATCGGTGT ACGATCTTTGGCTTTGGAACT 45.0 42.9 56.5 58.1 2994-3000 bp 0.909091 56.3
rpoC1_p4 CCCTAATTCACATCGGTGTCA ACGATCTTTGGCTTTGGAAC 47.6 45.0 57.7 56.6 2993-2999 bp 0.909091 56.0
rpoC1_p5 GACCCTAATTCACATCGGTGT ACGATCTTTGGCTTTGGAACT 47.6 42.9 57.7 58.1 2995-3000 bp 0.636364 46.9