Hotspot

matK

LSC · 2137-3651 · 1515 bp

Back to Fagus

Pi 0.0035
MarkerSeek score 53.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1515
variable_sites
19
pi
0.0035
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 AGTCGATCAACCCCTATGCT AGCCTGTTTTGACCGTATCG 50.0 50.0 58.2 58.3 1718 bp 0.909091 73.4
matK_p2 AGTCGATCAACCCCTATGCT AGAAATCCCCTATCCCTGCT 50.0 50.0 58.2 57.5 1667 bp 0.909091 71.5
matK_p3 AGTCGATCAACCCCTATGCT GCCTGTTTTGACCGTATCGT 50.0 50.0 58.2 58.6 1717 bp 0.909091 71.1
matK_p4 GTCGATCAACCCCTATGCTT AGCCTGTTTTGACCGTATCG 50.0 50.0 57.4 58.3 1717 bp 0.909091 70.1
matK_p5 GTCGATCAACCCCTATGCTT AGAAATCCCCTATCCCTGCT 50.0 50.0 57.4 57.5 1666 bp 0.909091 68.2