Hotspot

atpF

LSC · 11918-13233 · 1316 bp

Back to Fagus

Pi 0.00218
MarkerSeek score 56.00
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1316
variable_sites
10
pi
0.00218
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 TGCTCGAATGGTTACCATGA AGTCTATCTATATCTAAGAGGAGA 45.0 33.3 56.9 52.3 1414-1426 bp 1.0 41.0
atpF_p2 GCTCGAATGGTTACCATGAGT AGTCTATCTATATCTAAGAGGAGA 47.6 33.3 58.1 52.3 1413-1425 bp 1.0 41.0
atpF_p3 CTGCTCGAATGGTTACCATGA AGTCTATCTATATCTAAGAGGAGA 47.6 33.3 58.1 52.3 1415-1427 bp 1.0 41.0
atpF_p4 TCTGCTCGAATGGTTACCATG AGTCTATCTATATCTAAGAGGAGA 47.6 33.3 58.1 52.3 1416-1428 bp 1.0 41.0
atpF_p5 TGCTCGAATGGTTACCATGAG AGTCTATCTATATCTAAGAGGAGA 47.6 33.3 58.1 52.3 1414-1426 bp 1.0 41.0