Hotspot

ndhD-psaC

SSC · 121054-121183 · 130 bp

Back to Fagus

Pi 0.0668
MarkerSeek score 73.90
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
130
variable_sites
18
pi
0.0668
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 AGAAAATTAAGGAACCCCCA GGCATGAAACAACTCGAAGC 40.0 50.0 54.1 58.0 249-253 bp 1.0 55.5
ndhD-psaC_p2 AGAAAATTAAGGAACCCCCAA GGCATGAAACAACTCGAAGC 38.1 50.0 54.9 58.0 249-253 bp 1.0 54.7
ndhD-psaC_p3 AAGAAAATTAAGGAACCCCCA GGCATGAAACAACTCGAAGC 38.1 50.0 54.9 58.0 250-254 bp 1.0 54.6
ndhD-psaC_p4 GAAAGAAAATTAAGGAACCCCCA GGCATGAAACAACTCGAAGC 39.1 50.0 56.9 58.0 252-256 bp 1.0 54.4
ndhD-psaC_p5 AGAAAATTAAGGAACCCCCAAA GGCATGAAACAACTCGAAGC 36.4 50.0 55.8 58.0 249-253 bp 1.0 53.1