Hotspot

ndhF-rpl32

SSC · 115802-116766 · 965 bp

Back to Fagus

Pi 0.00631
MarkerSeek score 50.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
965
variable_sites
23
pi
0.00631
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGTGGATCAATCAAGTGGCC GCTACCCAATACCCCTCTCT 50.0 55.0 57.9 57.9 1219-1326 bp 0.909091 79.7
ndhF-rpl32_p2 ACTAGGAAAAGCCCACATGC GCTACCCAATACCCCTCTCT 50.0 55.0 58.2 57.9 1076-1183 bp 0.909091 79.7
ndhF-rpl32_p3 CCGTCGGAAAAAGGAGAAGT GCTACCCAATACCCCTCTCT 50.0 55.0 57.8 57.9 1038-1145 bp 0.909091 79.7
ndhF-rpl32_p4 GCTAGGTCGTGTGAACCAAA GCTACCCAATACCCCTCTCT 50.0 55.0 58.1 57.9 1454-1561 bp 0.909091 79.6
ndhF-rpl32_p5 GTGGATCAATCAAGTGGCCA GCTACCCAATACCCCTCTCT 50.0 55.0 58.2 57.9 1218-1325 bp 0.909091 79.5