Hotspot

psbE-petL

LSC · 67677-68996 · 1320 bp

Back to Fagus

Pi 0.00291
MarkerSeek score 56.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1320
variable_sites
15
pi
0.00291
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC CGTATCTTGCTCAACCCGAT 50.0 50.0 57.8 57.8 1432-1446 bp 1.0 78.1
psbE-petL_p2 ACGAATAACCAACCCGCAAT CGTATCTTGCTCAACCCGAT 45.0 50.0 58.2 57.8 1518-1532 bp 1.0 77.9
psbE-petL_p3 CGAATAACCAACCCGCAATG CGTATCTTGCTCAACCCGAT 50.0 50.0 57.8 57.8 1517-1531 bp 1.0 77.8
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC CGTATCTTGCTCAACCCGAT 55.0 50.0 58.2 57.8 1427-1441 bp 1.0 77.7
psbE-petL_p5 AACCCGCAATGAAAAGGGAA CGTATCTTGCTCAACCCGAT 45.0 50.0 58.3 57.8 1508-1522 bp 1.0 77.0