Hotspot

rbcL-psaI

LSC · 59513-62353 · 2841 bp

Back to Fagus

Pi 0.00337
MarkerSeek score 56.10
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
2841
variable_sites
34
pi
0.00337
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 AATGGAGTCCTGAGCTAGCT GGCAATATTAGGCCCACGAA 50.0 50.0 57.8 58.0 2971-2980 bp 1.0 79.7
rbcL-psaI_p2 GCTGCTGCTTGTGAAGTATG AAAGAAGCCATCGCAATTGC 50.0 45.0 57.5 58.0 2975-2984 bp 1.0 76.9
rbcL-psaI_p3 GCTGCTGCTTGTGAAGTATG GGCAATATTAGGCCCACGAA 50.0 50.0 57.5 58.0 2954-2963 bp 1.0 76.7
rbcL-psaI_p4 AATGGAGTCCTGAGCTAGCT CGCAATTGCCGGCAATATTA 50.0 45.0 57.8 57.6 2981-2990 bp 1.0 76.5
rbcL-psaI_p5 AATGGAGTCCTGAGCTAGCT AAAGAAGCCATCGCAATTGC 50.0 45.0 57.8 58.0 2992-3000 bp 0.727273 68.9