Hotspot

rpl16

LSC · 81640-82999 · 1360 bp

Back to Fagopyrum

Pi 0.0492

MarkerSeek score 82.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1360
variable_sites: 118
pi: 0.0492
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGCATATCGGCGATTGCTAG	ATCGAACTAACCGAACAGGC	50.0	50.0	58.0	58.0	1815-1907 bp	1.0	90.2
rpl16_p2	CATATCGGCGATTGCTAGCT	ATCGAACTAACCGAACAGGC	50.0	50.0	58.0	58.0	1813-1905 bp	1.0	90.2
rpl16_p3	GAGCATATCGGCGATTGCTA	ATCGAACTAACCGAACAGGC	50.0	50.0	58.0	58.0	1816-1908 bp	1.0	90.2
rpl16_p4	AGCATATCGGCGATTGCTAG	CTCAGGTGTCGAGGGAATTG	50.0	55.0	58.0	58.0	2169-2190 bp	0.5	62.0
rpl16_p5	CATATCGGCGATTGCTAGCT	CTCAGGTGTCGAGGGAATTG	50.0	55.0	58.0	58.0	2167-2188 bp	0.5	62.0