Hotspot

rpl32-trnL-UAG

SSC · 118545-119301 · 757 bp

Back to Fagopyrum

Pi 0.0933
MarkerSeek score 69.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
757
variable_sites
128
pi
0.0933
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.72
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTTTCGACCGGGAATGCTAA TCGAACCGAGATGCTTTAGC 50.0 50.0 58.0 58.1 763-943 bp 0.666667 77.9
rpl32-trnL-UAG_p2 TCGACCGGGAATGCTAAAAG TCGAACCGAGATGCTTTAGC 50.0 50.0 58.0 58.1 760-940 bp 0.666667 77.9
rpl32-trnL-UAG_p3 CTTTCGACCGGGAATGCTAA GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.0 58.1 1258-1427 bp 0.666667 77.8
rpl32-trnL-UAG_p4 TCGACCGGGAATGCTAAAAG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.0 58.1 1255-1424 bp 0.666667 77.8
rpl32-trnL-UAG_p5 TCGACCGGGAATGCTAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 734-914 bp 0.666667 77.5