Hotspot

trnP-UGG-psaJ

LSC · 66780-67082 · 303 bp

Back to Fagopyrum

Pi 0.0948

MarkerSeek score 69.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 54
pi: 0.0948
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGAACTGAACTAAGAGCGCT	AGAAAGGGCAGGGGATTAGT	50.0	50.0	58.0	58.0	851-993 bp	1.0	91.8
trnP-UGG-psaJ_p2	GTTTTGGAGACCCACGTTCT	AGAAAGGGCAGGGGATTAGT	50.0	50.0	58.0	58.0	873-1015 bp	1.0	91.6
trnP-UGG-psaJ_p3	CGAACTGAACTAAGAGCGCT	TCGTTACCCCCTTACCATGT	50.0	50.0	58.0	58.0	803-945 bp	0.833333	84.8
trnP-UGG-psaJ_p4	CGAACTGAACTAAGAGCGCT	TGTTAAAGCGTCCGGGAAAA	50.0	45.0	58.0	58.0	756-898 bp	0.5	62.8
trnP-UGG-psaJ_p5	GTTTTGGAGACCCACGTTCT	TGTTAAAGCGTCCGGGAAAA	50.0	45.0	58.0	58.0	778-920 bp	0.5	62.5