Hotspot

psbE-petL

LSC · 64973-65892 · 920 bp

Back to Fagopyrum

Pi 0.0559

MarkerSeek score 86.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 94
pi: 0.0559
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	973-1025 bp	1.0	76.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	968-1020 bp	1.0	76.3
psbE-petL_p3	CGCAAAAGAACGTTCTCCTG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.7	56.3	979-1031 bp	1.0	75.3
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.4	56.3	966-1018 bp	1.0	75.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	57.8	57.0	973-1025 bp	1.0	74.6