Hotspot

petA-psbJ

LSC · 63487-64183 · 697 bp

Back to Fagopyrum

Pi 0.0646

MarkerSeek score 84.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 87
pi: 0.0646
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	CACGGACTTGCTGTACCTAC	50.0	55.0	58.0	58.0	1391-1409 bp	1.0	88.4
petA-psbJ_p2	TTATTCCTCCAGGGCCAGAA	CACGGACTTGCTGTACCTAC	50.0	55.0	58.0	58.0	1400-1418 bp	1.0	88.3
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1360-1378 bp	1.0	88.3
petA-psbJ_p4	TTTATCCCGACGGAAGCAAG	CACGGACTTGCTGTACCTAC	50.0	55.0	58.0	58.0	1541-1559 bp	1.0	87.8
petA-psbJ_p5	TTTATCCCGACGGAAGCAAG	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1510-1528 bp	1.0	87.7