Hotspot

accD-psaI

LSC · 59100-59718 · 619 bp

Back to Fagopyrum

Pi 0.0536

MarkerSeek score 82.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 60
pi: 0.0536
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTATAGTGTGTGCTTCCGGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1093-1141 bp	1.0	89.0
accD-psaI_p2	TTATAGTGTGTGCTTCCGGC	ACTTGTTTTTCGAGACCCCC	50.0	50.0	58.0	58.0	1637-1683 bp	1.0	88.8
accD-psaI_p3	TTATAGTGTGTGCTTCCGGC	ACCATCCGGCCTAAACAATC	50.0	50.0	58.0	57.9	1144-1192 bp	1.0	88.6
accD-psaI_p4	GACATGGTCTCAACGGATCC	ACTTGTTTTTCGAGACCCCC	55.0	50.0	58.1	58.0	1884-1930 bp	1.0	87.7
accD-psaI_p5	TTATTTCCGAACCTAGCGCC	ACTTGTTTTTCGAGACCCCC	50.0	50.0	58.1	58.0	1454-1465 bp	0.5	61.1