Hotspot

rbcL-accD

LSC · 57204-57710 · 507 bp

Back to Fagopyrum

Pi 0.0592

MarkerSeek score 83.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 55
pi: 0.0592
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCAAATTTGAATTCCAAGCAATGG	GAATTAAACCACCATTTTTCCA	33.3	31.8	57.2	53.4	543-565 bp	1.0	51.9
rbcL-accD_p2	TCAAATTTGAATTCCAAGCAATGG	GAATTAAACCACCATTTTTCCAT	33.3	30.4	57.2	54.0	543-565 bp	1.0	51.9
rbcL-accD_p3	TCAAATTTGAATTCCAAGCAATGG	AATTAAACCACCATTTTTCCA	33.3	28.6	57.2	52.0	542-564 bp	1.0	51.9
rbcL-accD_p4	ATCAAATTTGAATTCCAAGCAATGG	GAATTAAACCACCATTTTTCCA	32.0	31.8	57.6	53.4	544-566 bp	1.0	51.9
rbcL-accD_p5	TCAAATTTGAATTCCAAGCAATGGA	GAATTAAACCACCATTTTTCCA	32.0	31.8	58.5	53.4	543-565 bp	1.0	51.9