Hotspot

atpB-rbcL

LSC · 55159-55775 · 617 bp

Back to Fagopyrum

Pi 0.0458

MarkerSeek score 80.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 54
pi: 0.0458
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TCATCTGCCCGTAGACTAGG	AGAAACGATCTCTCCAACGC	55.0	50.0	58.0	58.0	1990-2173 bp	1.0	84.6
atpB-rbcL_p2	CACTTCCATTCCTCGCGTTA	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1114-1297 bp	0.5	61.5
atpB-rbcL_p3	CACTTCCATTCCTCGCGTTA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1545-1728 bp	0.5	61.2
atpB-rbcL_p4	CTATTTTTCCTCCACGGCGA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1777-1960 bp	0.5	61.0
atpB-rbcL_p5	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1774-1957 bp	0.5	61.0