Hotspot

ndhC-trnV-UAC

LSC · 51273-52239 · 967 bp

Back to Fagopyrum

Pi 0.0732
MarkerSeek score 86.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
967
variable_sites
126
pi
0.0732
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAAATGCCCAGAAAATATCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 53.1 57.8 909-1128 bp 1.0 52.0
ndhC-trnV-UAC_p2 AGAAATGCCCAGAAAATATCAT AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.8 57.8 909-1128 bp 1.0 52.0
ndhC-trnV-UAC_p3 AGAAATGCCCAGAAAATATCA GAAGGTCTACGGTTCGAGTC 33.3 55.0 53.1 57.5 908-1127 bp 1.0 52.0
ndhC-trnV-UAC_p4 AGAAATGCCCAGAAAATATCA GTTCGAGTCCGTATAGCCCT 33.3 55.0 53.1 58.7 897-1116 bp 1.0 52.0
ndhC-trnV-UAC_p5 AGAAATGCCCAGAAAATATCA AAGGTCTACGGTTCGAGTCC 33.3 55.0 53.1 58.8 907-1126 bp 1.0 52.0