Hotspot

rps4-trnT-UGU

LSC · 46287-46858 · 572 bp

Back to Fagopyrum

Pi 0.0903

MarkerSeek score 84.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 91
pi: 0.0903
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	652-694 bp	1.0	90.1
rps4-trnT-UGU_p2	CCCCCAGACGTCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	621-663 bp	1.0	89.9
rps4-trnT-UGU_p3	TAAAACCCCCAGACGTCGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	657-699 bp	1.0	88.0
rps4-trnT-UGU_p4	TAAAACCCCCAGACGTCGTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.4	58.2	626-668 bp	1.0	87.7
rps4-trnT-UGU_p5	CCCCCAGACGTCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	57.9	57.5	660-702 bp	1.0	87.2