Hotspot

trnE-UUC-trnT-GGU

LSC · 30843-31386 · 544 bp

Back to Fagopyrum

Pi 0.0934

MarkerSeek score 83.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 92
pi: 0.0934
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TATGAGCGAATTCTTGGGCC	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	797-1002 bp	1.0	91.7
trnE-UUC-trnT-GGU_p2	TATGAGCGAATTCTTGGGCC	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	776-981 bp	1.0	91.2
trnE-UUC-trnT-GGU_p3	TATGAGCGAATTCTTGGGCC	TGGCGTTACTCTACCACTGA	50.0	50.0	58.0	58.1	773-978 bp	1.0	91.0
trnE-UUC-trnT-GGU_p4	TGTACGAGAGACACGAGTGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1143-1315 bp	0.833333	84.0
trnE-UUC-trnT-GGU_p5	TATGAGCGAATTCTTGGGCC	ATAGGGGATGCATCGATCGA	50.0	50.0	58.0	58.1	1458-1520 bp	0.5	64.1