Hotspot

trnD-GUC-trnY-GUA

LSC · 30185-30614 · 430 bp

Back to Fagopyrum

Pi 0.0693

MarkerSeek score 81.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 55
pi: 0.0693
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGG	50.0	55.0	58.5	58.9	445-490 bp	1.0	80.9
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TCTACGCTGGTTCAAATCCA	50.0	45.0	58.5	56.9	451-496 bp	1.0	79.1
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGG	50.0	52.6	58.5	57.8	444-489 bp	1.0	78.2
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GTCTACGCTGGTTCAAATCCA	50.0	47.6	58.5	58.3	452-497 bp	1.0	77.7
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	TGTCTACGCTGGTTCAAATCC	50.0	47.6	58.5	58.3	453-498 bp	1.0	77.7