Hotspot

trnK-UUU-rps16

LSC · 4278-5027 · 750 bp

Back to Fagopyrum

Pi 0.0619

MarkerSeek score 83.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 88
pi: 0.0619
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAACCTTTCCCGCATCAGT	TTTTCTTGAGCCGTACGAGG	45.0	50.0	58.0	57.9	1428-1464 bp	0.833333	83.7
trnK-UUU-rps16_p2	AAAACCTTTCCCGCATCAGT	GCCGTACGAGGAGAAAACTT	45.0	50.0	58.0	57.9	1419-1455 bp	0.833333	83.7
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	TAAAGAACTTCGCCCGACTG	55.0	50.0	58.0	57.9	907-916 bp	0.5	62.4
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TTTTCTTGAGCCGTACGAGG	55.0	50.0	58.0	57.9	1172-1181 bp	0.5	61.9
trnK-UUU-rps16_p5	AAAACCTTTCCCGCATCAGT	TAAAGAACTTCGCCCGACTG	45.0	50.0	58.0	57.9	1183 bp	0.333333	52.8