Hotspot

rps16

LSC · 4978-6147 · 1170 bp

Back to Fagaropsis

Pi 0.00428

MarkerSeek score 47.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1170
variable_sites: 5
pi: 0.00428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCCGTACTTAAAAGCCGAG	CGTGTCGGTCCAAATCTCAT	50.0	50.0	58.0	58.0	2356-2387 bp	1.0	81.5
rps16_p2	AGCCGTACTTAAAAGCCGAG	TGTGGTGAGCCCTTACCTTA	50.0	50.0	58.0	58.0	2044-2075 bp	1.0	81.5
rps16_p3	CCTTTCAGGATCAGTCGTGG	CGTGTCGGTCCAAATCTCAT	55.0	50.0	58.0	58.0	2440-2471 bp	1.0	81.3
rps16_p4	AGCCGTACTTAAAAGCCGAG	CATCATCCCCGCAGAAAGAA	50.0	50.0	58.0	58.0	2433-2464 bp	1.0	81.1
rps16_p5	AGCCGTACTTAAAAGCCGAG	TTCATCATCCCCGCAGAAAG	50.0	50.0	58.0	58.0	2435-2466 bp	1.0	81.1