Hotspot

ndhA

SSC · 122638-124868 · 2231 bp

Back to Fagaropsis

Pi 0.00272

MarkerSeek score 53.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2231
variable_sites: 6
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	45.0	50.0	55.4	57.9	2331-2355 bp	1.0	59.5
ndhA_p2	ACCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	45.0	50.0	55.4	57.8	2329-2353 bp	1.0	58.9
ndhA_p3	AACCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	42.9	50.0	56.3	57.9	2332-2356 bp	1.0	58.0
ndhA_p4	AACCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	42.9	50.0	56.3	57.8	2330-2354 bp	1.0	57.5
ndhA_p5	ACCAGTTACCATAGGGAACAT	ATGGGGGAAGTTGATCGTTG	42.9	50.0	56.0	57.9	2331-2355 bp	1.0	56.2