Hotspot

rpl14-rpl16

LSC · 83178-83304 · 127 bp

Back to Fagaropsis

Pi 0.00813

MarkerSeek score 60.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 1
pi: 0.00813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTATTGTCCGCTACATT	AATAGCGGCGTCAAAAATGC	50.0	45.0	58.1	58.1	219-223 bp	1.0	80.0
rpl14-rpl16_p2	CCGCTATTGTCCGCTACATT	TGAAATGAGTGGAGTCGCTG	50.0	50.0	58.1	57.9	264-268 bp	1.0	79.8
rpl14-rpl16_p3	CCGCTATTGTCCGCTACATT	GAAATGAGTGGAGTCGCTGA	50.0	50.0	58.1	57.6	263-267 bp	1.0	77.5
rpl14-rpl16_p4	CCGCTATTGTCCGCTACATT	TCGCTGAAAATATCGCTCGA	50.0	45.0	58.1	57.5	250-254 bp	1.0	76.6
rpl14-rpl16_p5	ACACATCAATTCTCGGGCAC	TGAAATGAGTGGAGTCGCTG	50.0	50.0	58.5	57.9	283-287 bp	1.0	76.4