Hotspot

ycf4-cemA

LSC · 62278-62754 · 477 bp

Back to Fagaropsis

Pi 0.0191

MarkerSeek score 68.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 9
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	CCACCGGGGCAAAAATACTA	45.0	50.0	58.1	57.9	586-591 bp	1.0	80.4
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GATCCACCGGGGCAAAAATA	45.0	50.0	58.1	58.2	589-594 bp	1.0	79.6
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	TAAGGGGAATGGATGCGTTC	45.0	50.0	58.1	57.7	542-547 bp	1.0	78.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	CTAAGGGGAATGGATGCGTT	45.0	50.0	58.1	57.7	543-548 bp	1.0	78.8
ycf4-cemA_p5	GGCCATTCCCTTGAATCGTA	CCACCGGGGCAAAAATACTA	50.0	50.0	57.7	57.9	653-658 bp	1.0	78.2