Hotspot

ndhC-trnV-UAC

LSC · 52709-53131 · 423 bp

Back to Fagaropsis

Pi 0.0118
MarkerSeek score 52.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
423
variable_sites
5
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCAAAATAGGAATAACGCTTGA AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.5 57.8 527-533 bp 1.0 43.3
ndhC-trnV-UAC_p2 ACCAAAATAGGAATAACGCTTGA GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.5 57.5 526-532 bp 1.0 40.8
ndhC-trnV-UAC_p3 ACCAAAATAGGAATAACGCTTG AGAAGGTCTACGGTTCGAGT 36.4 50.0 55.1 57.8 527-533 bp 1.0 40.8
ndhC-trnV-UAC_p4 ACCAAAATAGGAATAACGCTTGA GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.5 58.7 515-521 bp 1.0 40.8
ndhC-trnV-UAC_p5 ACCAAAATAGGAATAACGCTTGAT AGAAGGTCTACGGTTCGAGT 33.3 50.0 57.0 57.8 527-533 bp 1.0 40.8