Hotspot

rpoB-trnC-GCA

LSC · 27660-28883 · 1224 bp

Back to Fagaropsis

Pi 0.00409

MarkerSeek score 61.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 5
pi: 0.00409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	ATCGTTTGTTGATCAGGCGA	50.0	45.0	58.0	57.9	1335-1345 bp	1.0	79.5
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	ACCAGCCAAGCAGAATAAGG	50.0	50.0	58.0	57.9	1368-1378 bp	1.0	79.2
rpoB-trnC-GCA_p3	ATTCCCGCATTTCCATCTCC	GTTTGTTGATCAGGCGACAC	50.0	50.0	58.0	57.7	1332-1342 bp	1.0	77.7
rpoB-trnC-GCA_p4	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1294-1304 bp	1.0	77.4
rpoB-trnC-GCA_p5	ATTCCCGCATTTCCATCTCC	CAGCCAAGCAGAATAAGGGA	50.0	50.0	58.0	57.6	1366-1376 bp	1.0	76.8