Hotspot

trnS-GCU-trnS-CGA

LSC · 8299-9274 · 976 bp

Back to Fagaropsis

Pi 0.00617

MarkerSeek score 58.10

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 6
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CCGACGCTTTAATCCACTCA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.0	55.6	1079-1106 bp	1.0	61.8
trnS-GCU-trnS-CGA_p2	ACGGAAAGAGAGGGATTCGA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.2	55.6	1137-1164 bp	1.0	60.6
trnS-GCU-trnS-CGA_p3	CCGACGCTTTAATCCACTCA	AAACTGAAGGACCCCTTAACT	50.0	42.9	58.0	56.5	1080-1107 bp	1.0	60.3
trnS-GCU-trnS-CGA_p4	ACGCTTTAATCCACTCAGCC	AACTGAAGGACCCCTTAACT	50.0	45.0	58.3	55.6	1076-1103 bp	1.0	59.7
trnS-GCU-trnS-CGA_p5	CGCTTTAATCCACTCAGCCA	AACTGAAGGACCCCTTAACT	50.0	45.0	58.3	55.6	1075-1102 bp	1.0	59.7