Hotspot

rps16-trnQ-UUG

LSC · 6148-6969 · 822 bp

Back to Fagaropsis

Pi 0.00508

MarkerSeek score 65.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 4
pi: 0.00508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGAAAACGGTGGATGCAAAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	908-942 bp	1.0	81.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	861-895 bp	1.0	81.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTCG	45.0	50.0	58.1	58.1	919-953 bp	1.0	81.4
rps16-trnQ-UUG_p4	CGAAAACGGTGGATGCAAAA	GAAATTGAAATGGGGCGTCG	45.0	50.0	57.9	58.1	966-1000 bp	1.0	81.3
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	875-909 bp	1.0	79.8