Hotspot

rpl20-rps12

LSC · 73068-73828 · 761 bp

Back to Exochorda

Pi 0.0035

MarkerSeek score 61.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 4
pi: 0.0035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	AAATGTCACGAAATCCCCCG	55.0	50.0	58.2	58.5	922-924 bp	1.0	74.2
rpl20-rps12_p2	TAAGTCTTGAATGGGCCCCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.3	58.5	921-923 bp	1.0	73.6
rpl20-rps12_p3	GTAAGTCTTGAATGGGCCCC	CAGCGACGAGGAACATGTAC	55.0	55.0	58.2	58.7	885-887 bp	1.0	72.7
rpl20-rps12_p4	CCCTCGAAAGGTTGATGCAA	AAATGTCACGAAATCCCCCG	50.0	50.0	58.5	58.5	905-907 bp	1.0	72.4
rpl20-rps12_p5	TAAGTCTTGAATGGGCCCCT	CAGCGACGAGGAACATGTAC	50.0	55.0	58.3	58.7	884-886 bp	1.0	72.1