Hotspot

psbF-psbE

LSC · 68384-68392 · 9 bp

Back to Exochorda

Pi 0.0741

MarkerSeek score 75.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	ACGGTAGGTACAGCTAGTCC	AAAGCCGACAAGGAATTCCA	55.0	45.0	58.0	57.7	163 bp	1.0	77.8
psbF-psbE_p2	GGTAGGTACAGCTAGTCCGT	AAAGCCGACAAGGAATTCCA	55.0	45.0	58.0	57.7	161 bp	1.0	77.8
psbF-psbE_p3	GTCCGTGAACAGCTAACCAT	AAAGCCGACAAGGAATTCCA	50.0	45.0	57.9	57.7	147 bp	1.0	77.5
psbF-psbE_p4	AGCTAGTCCGTGAACAGCTA	AAAGCCGACAAGGAATTCCA	50.0	45.0	58.2	57.7	152 bp	1.0	76.9
psbF-psbE_p5	TGGATGAACTGCATTGCTGA	AAAGCCGACAAGGAATTCCA	45.0	45.0	57.8	57.7	202 bp	1.0	76.5