Hotspot

accD-psaI

LSC · 62849-63726 · 878 bp

Back to Exochorda

Pi 0.00407

MarkerSeek score 65.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 5
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTGTTTCGGCTACATGCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.8	58.0	937-995 bp	1.0	82.7
accD-psaI_p2	AGTTGTTTCGGCTACATGCT	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.8	58.0	928-986 bp	1.0	82.6
accD-psaI_p3	AGTTGTTTCGGCTACATGCT	AAATGGAGGGGAAGCTGTTG	45.0	50.0	57.8	58.1	895-953 bp	1.0	82.4
accD-psaI_p4	AGTTGTTTCGGCTACATGCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	943-1001 bp	1.0	82.1
accD-psaI_p5	TTCTGAGCGAGTTGTTTCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.5	58.0	946-1004 bp	1.0	80.2