Hotspot

ndhC-trnV-UAC

LSC · 54995-55467 · 473 bp

Back to Exochorda

Pi 0.00564

MarkerSeek score 60.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 4
pi: 0.00564
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGTTAATCGGGGCT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	669-682 bp	1.0	79.7
ndhC-trnV-UAC_p2	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	605-618 bp	1.0	79.6
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCAGAAA	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.8	58.2	656-669 bp	1.0	79.2
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	592-605 bp	1.0	79.1
ndhC-trnV-UAC_p5	GTCCTTTGTTAATCGGGGCT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	671-684 bp	1.0	78.5