Hotspot

trnF-GAA-ndhJ

LSC · 52437-53311 · 875 bp

Back to Exochorda

Pi 0.0061

MarkerSeek score 62.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 8
pi: 0.0061
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	1048-1077 bp	1.0	80.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	1012-1041 bp	1.0	79.9
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	1036-1065 bp	1.0	79.2
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	1061-1090 bp	1.0	79.1
trnF-GAA-ndhJ_p5	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	1058-1087 bp	1.0	79.0