Hotspot

rpoB-trnC-GCA

LSC · 28049-29262 · 1214 bp

Back to Exochorda

Pi 0.00166

MarkerSeek score 60.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 3
pi: 0.00166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCACCCCCAAGCATTT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.0	58.1	1339-1348 bp	1.0	79.7
rpoB-trnC-GCA_p2	GACTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.2	58.1	1344-1353 bp	1.0	78.8
rpoB-trnC-GCA_p3	TTCCACCCCCAAGCATTTTT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.2	58.1	1337-1346 bp	1.0	78.6
rpoB-trnC-GCA_p4	TTTCCACCCCCAAGCATTTT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.2	58.1	1338-1347 bp	1.0	78.6
rpoB-trnC-GCA_p5	ATTTCCACCCCCAAGCATTT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	58.0	58.4	1321-1330 bp	1.0	77.5