Hotspot

trnR-UCU-atpA

LSC · 10398-11003 · 606 bp

Back to Exochorda

Pi 0.0033

MarkerSeek score 63.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 3
pi: 0.0033
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	TTTACCGACGAAGCAGAAGC	42.9	50.0	57.4	58.6	770-796 bp	1.0	64.5
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	TTTACCGACGAAGCAGAAGC	42.9	50.0	57.4	58.6	769-795 bp	1.0	64.5
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTTACCGACGAAGCAGAAGC	47.6	50.0	57.3	58.6	709-735 bp	1.0	63.7
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATTG	ACATTTACCGACGAAGCAGA	42.9	45.0	57.4	57.3	773-799 bp	1.0	63.2
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCA	42.9	45.0	57.4	57.3	775-801 bp	1.0	63.2