Hotspot

rps16-trnQ-UUG

LSC · 6597-7426 · 830 bp

Back to Exochorda

Pi 0.00402

MarkerSeek score 61.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 5
pi: 0.00402
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	903-947 bp	1.0	78.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GACGAACAACCAGTCCCAAT	45.0	50.0	58.1	58.1	999-1043 bp	1.0	78.4
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	914-958 bp	1.0	78.2
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GACGAACAACCAGTCCCAAT	50.0	50.0	58.2	58.1	1010-1054 bp	1.0	77.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTGTCGACTAGAAATGGGGC	45.0	55.0	58.1	58.3	965-1009 bp	1.0	76.5