primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6597	7426	903-947	1.000000	78.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GACGAACAACCAGTCCCAAT	20	20	45.0	50.0	58.1	58.1	8.1	0.0	0.247115	6597	7426	999-1043	1.000000	78.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6597	7426	914-958	1.000000	78.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GACGAACAACCAGTCCCAAT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.312023	6597	7426	1010-1054	1.000000	77.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTGTCGACTAGAAATGGGGC	20	20	45.0	55.0	58.1	58.3	8.1	17.9	0.486173	6597	7426	965-1009	1.000000	76.5
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TGGAATGAAAAGCGTCCATTG	TTTACCGACGAAGCAGAAGC	21	20	42.9	50.0	57.4	58.6	5.3	0.0	2.148871	10398	11003	770-796	1.000000	64.5
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGAATGAAAAGCGTCCATTGT	TTTACCGACGAAGCAGAAGC	21	20	42.9	50.0	57.4	58.6	5.3	0.0	2.150553	10398	11003	769-795	1.000000	64.5
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	TTTACCGACGAAGCAGAAGC	21	20	47.6	50.0	57.3	58.6	0.0	0.0	2.267379	10398	11003	709-735	1.000000	63.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGAATGAAAAGCGTCCATTG	ACATTTACCGACGAAGCAGA	21	20	42.9	45.0	57.4	57.3	5.3	0.0	2.310427	10398	11003	773-799	1.000000	63.2
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCA	21	20	42.9	45.0	57.4	57.3	5.3	0.0	2.310427	10398	11003	775-801	1.000000	63.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14573	15725	1209-1211	1.000000	80.0
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14573	15725	1238-1240	1.000000	79.1
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14573	15725	1210-1212	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	14573	15725	1239-1241	1.000000	78.0
atpH-atpI_p5	atpH-atpI	5	AATAGAAGCAAGTCCGACGG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	57.7	58.0	7.8	20.7	0.322381	14573	15725	1243-1245	1.000000	77.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTTCCACCCCCAAGCATTT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.125435	28049	29262	1339-1348	1.000000	79.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GACTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.242704	28049	29262	1344-1353	1.000000	78.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTCCACCCCCAAGCATTTTT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.269026	28049	29262	1337-1346	1.000000	78.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTTCCACCCCCAAGCATTTT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.269026	28049	29262	1338-1347	1.000000	78.6
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTTCCACCCCCAAGCATTT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	58.0	58.4	0.0	14.6	0.398798	28049	29262	1321-1330	1.000000	77.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	34374	35884	1660-1679	1.000000	81.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	34374	35884	1663-1682	1.000000	81.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	34374	35884	1662-1681	1.000000	81.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	34374	35884	1665-1684	1.000000	81.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.293100	34374	35884	1637-1656	1.000000	79.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.9	57.6	4.1	18.7	0.471060	44987	45724	890-898	1.000000	76.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	20	20	50.0	55.0	57.9	58.6	4.1	18.9	0.741772	44987	45724	930-938	1.000000	74.5
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCTATAGCGCT	20	20	50.0	45.0	57.9	57.3	4.1	17.3	0.806751	44987	45724	899-907	1.000000	74.0
psaA-ycf3_p4	psaA-ycf3	4	TTGAGAAATGACCCGGTCTG	GCTATAGCGCTTACTCCTGG	20	20	50.0	55.0	57.5	57.6	18.5	18.7	0.828412	44987	45724	910-918	1.000000	73.8
psaA-ycf3_p5	psaA-ycf3	5	TTTAACTTCTGGTTCCGGCG	GCTATAGCGCTTACTCCTGG	20	20	50.0	55.0	58.5	57.6	0.0	18.7	0.840636	44987	45724	843-851	1.000000	73.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50088	51344	1283-1307	1.000000	80.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50088	51344	1291-1315	1.000000	80.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50088	51344	1315-1339	1.000000	79.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATGGAGAAAAGCCGGCTATC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	33.1	0.0	0.267047	50088	51344	1335-1359	1.000000	79.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	50088	51344	1323-1347	1.000000	79.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.066573	52437	53311	1048-1077	1.000000	80.2
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	52437	53311	1012-1041	1.000000	79.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.200366	52437	53311	1036-1065	1.000000	79.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.199732	52437	53311	1061-1090	1.000000	79.1
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.220411	52437	53311	1058-1087	1.000000	79.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGTTAATCGGGGCT	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.289156	54995	55467	669-682	1.000000	79.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.321560	54995	55467	605-618	1.000000	79.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CGGGGCTAAAACTCCAGAAA	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.359081	54995	55467	656-669	1.000000	79.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391484	54995	55467	592-605	1.000000	79.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTTGTTAATCGGGGCT	ATGCACGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.437922	54995	55467	671-684	1.000000	78.5
atpB-rbcL_p1	atpB-rbcL	1	CCCCAGGACCAGACGTAG	CACCAGCTTTGAATCCAACAC	18	21	66.7	47.6	58.4	58.0	0.0	0.0	3.399897	58510	59290	864-864	1.000000	53.3
atpB-rbcL_p2	atpB-rbcL	2	CCCCAGGACCAGACGTAG	ACCAGCTTTGAATCCAACACT	18	21	66.7	42.9	58.4	58.1	0.0	0.0	3.433185	58510	59290	863-863	1.000000	53.0
atpB-rbcL_p3	atpB-rbcL	3	CCCCAGGACCAGACGTAG	ACACCAGCTTTGAATCCAACA	18	21	66.7	42.9	58.4	58.3	0.0	0.0	3.717467	58510	59290	865-865	1.000000	50.7
atpB-rbcL_p4	atpB-rbcL	4	CCCCAGGACCAGACGTAG	AGTCTCTGTTTGTGGTGACA	18	20	66.7	45.0	58.4	56.6	0.0	0.0	3.754328	58510	59290	836-836	1.000000	50.4
atpB-rbcL_p5	atpB-rbcL	5	CCCCAGGACCAGACGTAG	GCTTTGAATCCAACACTTGCT	18	21	66.7	42.9	58.4	57.6	0.0	0.0	3.758414	58510	59290	859-859	1.000000	50.4
accD-psaI_p1	accD-psaI	1	AGTTGTTTCGGCTACATGCT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.8	58.0	0.0	26.9	0.212007	62849	63726	937-995	1.000000	82.7
accD-psaI_p2	accD-psaI	2	AGTTGTTTCGGCTACATGCT	TTGCCGGAAATACTAAGCCC	20	20	45.0	50.0	57.8	58.0	0.0	5.0	0.232006	62849	63726	928-986	1.000000	82.6
accD-psaI_p3	accD-psaI	3	AGTTGTTTCGGCTACATGCT	AAATGGAGGGGAAGCTGTTG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.256136	62849	63726	895-953	1.000000	82.4
accD-psaI_p4	accD-psaI	4	AGTTGTTTCGGCTACATGCT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.8	57.9	0.0	26.8	0.288541	62849	63726	943-1001	1.000000	82.1
accD-psaI_p5	accD-psaI	5	TTCTGAGCGAGTTGTTTCGG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.5	58.0	0.0	26.9	0.529013	62849	63726	946-1004	1.000000	80.2
psbF-psbE_p1	psbF-psbE	1	ACGGTAGGTACAGCTAGTCC	AAAGCCGACAAGGAATTCCA	20	20	55.0	45.0	58.0	57.7	0.0	26.2	0.336253	68384	68392	163-163	1.000000	77.8
psbF-psbE_p2	psbF-psbE	2	GGTAGGTACAGCTAGTCCGT	AAAGCCGACAAGGAATTCCA	20	20	55.0	45.0	58.0	57.7	0.0	26.2	0.336253	68384	68392	161-161	1.000000	77.8
psbF-psbE_p3	psbF-psbE	3	GTCCGTGAACAGCTAACCAT	AAAGCCGACAAGGAATTCCA	20	20	50.0	45.0	57.9	57.7	0.0	26.2	0.381127	68384	68392	147-147	1.000000	77.5
psbF-psbE_p4	psbF-psbE	4	AGCTAGTCCGTGAACAGCTA	AAAGCCGACAAGGAATTCCA	20	20	50.0	45.0	58.2	57.7	8.0	26.2	0.450870	68384	68392	152-152	1.000000	76.9
psbF-psbE_p5	psbF-psbE	5	TGGATGAACTGCATTGCTGA	AAAGCCGACAAGGAATTCCA	20	20	45.0	45.0	57.8	57.7	20.7	26.2	0.489835	68384	68392	202-202	1.000000	76.5
petL-petG_p1	petL-petG	1	TTGCGGGATGGGTAAATGTC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.2	58.0	0.0	2.6	0.258345	70036	70196	807-807	1.000000	78.7
petL-petG_p2	petL-petG	2	TTGCGGGATGGGTAAATGTC	GGATGTAGCGCAGTTTGGTA	20	20	50.0	50.0	58.2	58.0	0.0	11.5	0.260860	70036	70196	1018-1018	1.000000	78.6
petL-petG_p3	petL-petG	3	TTGCGGGATGGGTAAATGTC	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.279146	70036	70196	785-785	1.000000	78.6
petL-petG_p4	petL-petG	4	TTGCGGGATGGGTAAATGTC	GGAAGTAGGAGTAGGGGGTC	20	20	50.0	60.0	58.2	57.9	0.0	0.0	0.327436	70036	70196	641-641	1.000000	78.4
petL-petG_p5	petL-petG	5	TTGCGGGATGGGTAAATGTC	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.2	57.9	0.0	1.4	0.331267	70036	70196	771-771	1.000000	78.2
rpl20-rps12_p1	rpl20-rps12	1	GTAAGTCTTGAATGGGCCCC	AAATGTCACGAAATCCCCCG	20	20	55.0	50.0	58.2	58.5	22.3	0.0	0.776582	73068	73828	922-924	1.000000	74.2
rpl20-rps12_p2	rpl20-rps12	2	TAAGTCTTGAATGGGCCCCT	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.3	58.5	22.3	0.0	0.859321	73068	73828	921-923	1.000000	73.6
rpl20-rps12_p3	rpl20-rps12	3	GTAAGTCTTGAATGGGCCCC	CAGCGACGAGGAACATGTAC	20	20	55.0	55.0	58.2	58.7	22.3	0.0	0.966866	73068	73828	885-887	1.000000	72.7
rpl20-rps12_p4	rpl20-rps12	4	CCCTCGAAAGGTTGATGCAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.5	58.5	0.0	0.0	1.006925	73068	73828	905-907	1.000000	72.4
rpl20-rps12_p5	rpl20-rps12	5	TAAGTCTTGAATGGGCCCCT	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	58.3	58.7	22.3	0.0	1.049605	73068	73828	884-886	1.000000	72.1
clpP_p1	clpP	1	AATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACA	20	20	45.0	45.0	56.2	56.9	0.0	0.0	2.877613	74108	76187	2172-2182	1.000000	57.3
clpP_p2	clpP	2	AATCCCAAATCACGGATTCG	ACATCAAAGCGGGATACAGT	20	20	45.0	45.0	56.2	56.9	0.0	0.0	2.888217	74108	76187	2170-2180	1.000000	57.2
clpP_p3	clpP	3	AATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACAG	20	21	45.0	47.6	56.2	58.1	0.0	0.0	2.902716	74108	76187	2172-2182	1.000000	57.1
clpP_p4	clpP	4	AATCCCAAATCACGGATTCG	GCTTTCACATCAAAGCGGGA	20	20	45.0	50.0	56.2	59.1	0.0	2.4	2.926673	74108	76187	2176-2186	1.000000	56.9
clpP_p5	clpP	5	AAATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACA	21	20	42.9	45.0	56.9	56.9	2.0	0.0	3.125435	74108	76187	2173-2183	1.000000	55.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GTAAAGGGTATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.145640	118165	119714	1674-1723	1.000000	80.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GTAAAGGGTATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.147011	118165	119714	1675-1724	1.000000	80.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAGGGTATTGGGCAGCATT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.260366	118165	119714	1672-1721	1.000000	79.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAGGGTATTGGGCAGCATT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.261736	118165	119714	1673-1722	1.000000	79.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GTAAAGGGTATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.262087	118165	119714	1684-1733	1.000000	79.2
ycf1_p1	ycf1	1	TCAGTCTAAGCAGGAGACAA	GCAGGCCAAGCAGATCTTAA	20	20	45.0	50.0	55.5	58.2	13.2	14.6	2.730866	130243	130362	235-235	1.000000	59.2
ycf1_p2	ycf1	2	ATCAGTCTAAGCAGGAGACA	GCAGGCCAAGCAGATCTTAA	20	20	45.0	50.0	55.3	58.2	13.2	14.6	2.968854	130243	130362	236-236	1.000000	57.3
ycf1_p3	ycf1	3	TCAGTCTAAGCAGGAGACAA	GGAAAAAGAATCTGCAGGCC	20	20	45.0	50.0	55.5	57.3	13.2	15.1	3.148052	130243	130362	248-248	1.000000	55.8
ycf1_p4	ycf1	4	TCAGTCTAAGCAGGAGACAA	GGGAAAAAGAATCTGCAGGC	20	20	45.0	50.0	55.5	57.3	13.2	15.1	3.148052	130243	130362	249-249	1.000000	55.8
ycf1_p5	ycf1	5	TCAGTCTAAGCAGGAGACAAT	GCAGGCCAAGCAGATCTTAA	21	20	42.9	50.0	56.1	58.2	13.2	14.6	3.152910	130243	130362	235-235	1.000000	55.8