Hotspot

ycf4

LSC · 64252-64806 · 555 bp

Back to Exbucklandia

Pi 0.0036

MarkerSeek score 40.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TGAGAGTTACTTCGGAAACACA	CGAATGAGCGCTCTGAAGAA	40.9	50.0	57.3	58.4	735 bp	1.0	56.1
ycf4_p2	TGAGAGTTACTTCGGAAACACA	AGCGCTCTGAAGAAACTTCA	40.9	45.0	57.3	57.5	729 bp	1.0	54.8
ycf4_p3	TGAGAGTTACTTCGGAAACACA	TGAGCGCTCTGAAGAAACTT	40.9	45.0	57.3	57.5	731 bp	1.0	54.8
ycf4_p4	AGGTTCACATCAGAATAGTGCT	CGAATGAGCGCTCTGAAGAA	40.9	50.0	57.2	58.4	763 bp	1.0	54.7
ycf4_p5	ACTTCGGAAACACAAAGAGT	CGAATGAGCGCTCTGAAGAA	40.0	50.0	55.1	58.4	727 bp	1.0	54.5