Hotspot

rps2

LSC · 16914-17648 · 735 bp

Back to Exbucklandia

Pi 0.00272

MarkerSeek score 33.20

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 735
variable_sites: 3
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGAACCCAAGGACCGTATTC	TTGGATTGATGCGAGCAGAA	55.0	45.0	58.0	57.9	1843-1844 bp	1.0	79.2
rps2_p2	GGAACCCAAGGACCGTATTC	AGGCCTGAAAGAGAACGTTG	55.0	50.0	58.0	58.1	1659-1660 bp	1.0	79.2
rps2_p3	GGAACCCAAGGACCGTATTC	AAAGAGGAAGTGTGGGGAGA	55.0	50.0	58.0	57.9	1226 bp	1.0	79.2
rps2_p4	GGAACCCAAGGACCGTATTC	AAGAGGAAGTGTGGGGAGAA	55.0	50.0	58.0	57.9	1225 bp	1.0	79.2
rps2_p5	GGAACCCAAGGACCGTATTC	ACCGCTCGAGTTTTAGCAAA	55.0	45.0	58.0	58.1	1712-1713 bp	1.0	79.1