Hotspot

rps16

LSC · 5260-6361 · 1102 bp

Back to Exbucklandia

Pi 0.00181

MarkerSeek score 59.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1102
variable_sites: 3
pi: 0.00181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATACGAGTCATCCCCACCC	ACCGGTTCCAAACTAGAGGA	55.0	50.0	58.0	58.0	1167-1169 bp	1.0	80.1
rps16_p2	AGTCATCCCCACCCTCTTTT	ACCGGTTCCAAACTAGAGGA	50.0	50.0	58.2	58.0	1161-1163 bp	1.0	78.3
rps16_p3	GTCATCCCCACCCTCTTTTT	ACCGGTTCCAAACTAGAGGA	50.0	50.0	57.4	58.0	1160-1162 bp	1.0	75.3
rps16_p4	GAGTCATCCCCACCCTCTTT	ACCGGTTCCAAACTAGAGGA	55.0	50.0	58.7	58.0	1162-1164 bp	1.0	74.5
rps16_p5	GAGTCATCCCCACCCTCTT	ACCGGTTCCAAACTAGAGGA	57.9	50.0	58.0	58.0	1162-1164 bp	1.0	72.1