Hotspot

psaC

SSC · 123108-123353 · 246 bp

Back to Exbucklandia

Pi 0.00271

MarkerSeek score 31.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	ATCAAATGGATTCGCGTGGA	TGACGTTCAAAAATTTGTAGATCCA	45.0	32.0	58.0	57.7	306 bp	1.0	40.3
psaC_p2	AAATGGATTCGCGTGGAGTT	TGACGTTCAAAAATTTGTAGATCCA	45.0	32.0	58.2	57.7	303 bp	1.0	40.3
psaC_p3	AATGGATTCGCGTGGAGTTT	TGACGTTCAAAAATTTGTAGATCCA	45.0	32.0	58.2	57.7	302 bp	1.0	40.3
psaC_p4	ATGGATTCGCGTGGAGTTTT	TGACGTTCAAAAATTTGTAGATCCA	45.0	32.0	58.2	57.7	301 bp	1.0	40.3
psaC_p5	TTTTCGAGCACGGGTTTTTG	TGACGTTCAAAAATTTGTAGATCCA	45.0	32.0	57.8	57.7	338 bp	1.0	40.2