Hotspot

trnL-CAA-ycf15

IRa · 150648-151270 · 623 bp

Back to Exbucklandia

Pi 0.00535

MarkerSeek score 62.20

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 623
variable_sites: 5
pi: 0.00535
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.0	57.9	736 bp	1.0	79.6
trnL-CAA-ycf15_p2	AAATGGTAGACACGCGAGAC	TGGTTGTTGCTATCTGCTCC	50.0	50.0	58.0	57.9	737 bp	1.0	79.6
trnL-CAA-ycf15_p3	TGAAATGGTAGACACGCGAG	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.0	57.9	738 bp	1.0	79.6
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	TGGTTGTTGCTATCTGCTCC	50.0	50.0	58.0	57.9	739 bp	1.0	79.6
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	GGTTGTTGCTATCTGCTCCA	50.0	50.0	58.1	57.9	748 bp	1.0	79.0