Hotspot

rpl32-trnL-UAG

SSC · 118917-120155 · 1239 bp

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Pi 0.00379
MarkerSeek score 62.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1239
variable_sites
7
pi
0.00379
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCGTTAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1355-1365 bp 1.0 80.8
rpl32-trnL-UAG_p2 GGGATATTGGGCAGCGTTAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1356-1366 bp 1.0 80.8
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCGTTAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1365-1375 bp 1.0 79.9
rpl32-trnL-UAG_p4 GGGATATTGGGCAGCGTTAA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 1364-1374 bp 1.0 79.3
rpl32-trnL-UAG_p5 GGGATATTGGGCAGCGTTAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1363-1373 bp 1.0 79.3