Hotspot

rpl20-rps12

LSC · 73783-74553 · 771 bp

Back to Exbucklandia

Pi 0.00346

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 4
pi: 0.00346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	845 bp	1.0	76.7
rpl20-rps12_p2	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	843 bp	1.0	76.3
rpl20-rps12_p3	TCCTCGTCGAATTCTGGTCA	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	819 bp	1.0	75.8
rpl20-rps12_p4	TTGTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	844 bp	1.0	75.7
rpl20-rps12_p5	CCTCGTCGAATTCTGGTCAT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.4	57.9	818 bp	1.0	74.7