Hotspot

trnC-GCA-petN

LSC · 29525-30379 · 855 bp

Back to Exbucklandia

Pi 0.00312

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 4
pi: 0.00312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGTCCACCTCTTCCCCATAC	45.0	55.0	57.9	58.1	1032-1036 bp	1.0	79.2
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	GTCCACCTCTTCCCCATACT	45.0	55.0	57.9	58.1	1031-1035 bp	1.0	79.2
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGTCCACCTCTTCCCCATAC	50.0	55.0	58.1	58.1	976-980 bp	1.0	79.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GTCCACCTCTTCCCCATACT	50.0	55.0	58.1	58.1	975-979 bp	1.0	79.0
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	973-977 bp	1.0	79.0